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Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae
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JoVE Journal Medicina
Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae
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Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae

DOI:
10.3791/63259-v

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09:43 min

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November 22, 2021

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, , , , ,
1Centre for Discovery Brain Sciences,University of Edinburgh Medical School: Biomedical Sciences, 2Center for Regenerative Therapies at the TU Dresden

Capitoli

  • 00:04Introduction
  • 00:51Sample Preparation and the VAST Software Set Up
  • 02:05Set Up ImageJ/Fiji for Laser Lesions and the Python IDE
  • 02:53Performing Laser Lesions on the VAST System
  • 06:29Results: Laser Lesions as an Alternative Tool to Manual Lesions for Studies on Spinal Cord Regeneration
  • 08:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

The present protocol describes a method to induce tissue-specific and highly reproducible injuries in zebrafish larvae using a laser lesion system combined with an automated microfluidic platform for larvae handling.

Tags

Keywords: Laser-induced InjuriesSpinal Cord RegenerationZebrafish LarvaeSemi-automated TechniqueVAST EquipmentAnesthetizing LarvaeAutomated Zebrafish ImagingLaser AblationPython IDEScript ManagementPlate HandlingMicroscope FocusingReproducibility

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