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Biochemistry

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JoVE Journal Biochemistry
Rening av endogena Drosophila transienta receptorpotentialkanaler
 
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Rening av endogena Drosophila transienta receptorpotentialkanaler

Article DOI: 10.3791/63260-v • 08:39 min • December 28th, 2021
December 28th, 2021 •
*1, *1, 1, 1, 1, 1
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center
* These authors contributed equally

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Baserat på monteringsmekanismen för INAD-proteinkomplexet utvecklades i detta protokoll en modifierad affinitetsrening plus konkurrensstrategi för att rena den endogena Drosophila TRP-kanalen.

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Biokemi utgåva 178 proteinrening Drosophila TRP-kanal INAD
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