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亲和色谱法表达和纯化人基质金属蛋白酶-3

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

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亲和色谱法表达和纯化人基质金属蛋白酶-3

DOI:

10.3791/63263-v

•

07:32 min

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March 30, 2022

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Alexander J. Bolt*¹, Linh D. Do*¹, Maryam Raeeszadeh-Sarmazdeh

¹Department of Chemical & Materials Engineering,University of Nevada, Reno

Capitoli

00:04Introduction
00:52MMP Expression
03:46MMP Purification
04:27MMP Refolding
05:12MMP Activation
05:46Results: Purification and Activation of Hisx6-Pro-MMP-3cd From BL21(DE3) and R2DP Cells
06:38Conclusion

Summary

Traduzione automatica

His-tag纯化，透析和活化用于提高细菌中可溶性活性基质金属蛋白酶-3催化结构域蛋白表达的产量。蛋白质级分通过SDS-PAGE凝胶进行分析。

Tags

Keywords: Bacterial Expression Recombinant MMP-3 Affinity Chromatography E. Coli R2DP Cell Strain MMP Inhibitors Protein Purification Protein Folding Post-translational Processing IPTG Induction Cell Lysis Inclusion Body Solubilization Sonication Centrifugation

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