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用于微生物化学和蛋白质生产的光控发酵
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Bioingegneria
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JoVE Journal Bioingegneria
Light-Controlled Fermentations for Microbial Chemical and Protein Production
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用于微生物化学和蛋白质生产的光控发酵

DOI:
10.3791/63269-v

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08:37 min

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March 22, 2022

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, ,
1Department of Chemical and Biological Engineering,Princeton University, 2The Andlinger Center for Energy and the Environment,Princeton University, 3Department of Molecular Biology,Princeton University, 4High Meadows Environmental Institute,Princeton University

Capitoli

  • 00:04Introduction
  • 00:43Light-Controlled Chemical Production Using the Saccharomyces cerevisiae OptoINVRT7 Circuit
  • 05:12Three-Phase Fermentation Using the Saccharomyces cerevisiae OptoAMP System
  • 06:26Results: The Effects of Light-Regulated Fermentations for Chemical and Protein Production
  • 08:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

微生物代谢的光遗传学控制为发酵过程提供了灵活的动态控制。这里的实验方案展示了如何建立蓝光调节的发酵，用于不同体积尺度的化学和蛋白质生产。

Tags

OptogeneticsLight-controlled FermentationMicrobial Chemical ProductionMicrobial Protein ProductionSaccharomyces CerevisiaeOptoINVRTPIGA1 PromoterBlue LightGenetic EngineeringBioprocessingBiomanufacturing

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