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Capitoli
- 00:04Introduction
- 01:19Equipment Setup and DiI Perfusion
- 04:08Preparation of Footpad Tissue for Confocal Laser Scanning Microscopy
- 05:01Confocal Laser Scanning Microscopy
- 06:37Results: Anatomy of the Murine Hindlimb Vasculature
- 07:20Conclusion
The present protocol describes a unique, clinically relevant model of peripheral arterial disease that combines femoral artery and vein electrocoagulation with the administration of a nitric oxide synthase inhibitor to induce hindlimb gangrene in FVB mice. Intracardiac DiI perfusion is then used for high-resolution, three-dimensional imaging of the footpad vasculature.
