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Developmental Biology

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JoVE Journal Developmental Biology
Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders
 

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

Article DOI: 10.3791/63311-v • 10:31 min • February 9th, 2022
February 9th, 2022 •
1,2, 1, 1,2
1Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), 2College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation

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The protocol allows the generation of a pure adipocyte population from induced pluripotent stem cells (iPSCs). Retinoic acid is used to differentiate iPSCs into mesenchymal stem cells (MSCs) which are used for producing adipocytes. Then, a sorting approach based on Nile red staining is used to obtain pure adipocytes.

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Robust Differentiation Human IPSCs Pure Population Adipocytes Adipocyte-associated Disorders Mesenchymal Stem Cells Nile Red Sorting Protocol Rapidly Proliferating Mature Adipocytes Dissociation Medium StemFlex Media MSC Differentiation Media Rock Inhibitor Embryonic Bodies
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