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Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes
 

Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

Article DOI: 10.3791/63329-v • 11:47 min • March 4th, 2022
March 4th, 2022 •
1, 1, 1, 1
1Department of Medicine, Division of Nephrology, Indiana University School of Medicine

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Here, we present a protocol using 2-photon microscopy in Munich Wistar Fromter rats with surface glomeruli to quantifythe effects of prolonged ureteral obstruction on glomerular dynamics and function.

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2-Photon Microscopy Chronic Unilateral Ureteral Obstruction Glomerular Processes Kidney Function Inflammation Fibrosis Pathophysiologic Mechanisms Cell-cell Interactions Subcellular Events Structure And Function Anesthetize Incision Renal Pedicle Renal Artery Renal Vein Ureter Forceps Suture Complete Obstruction Muscle Layers Surgical Staples Surgical Preparation Indwelling Venous Access Line
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