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从微解剖小鼠脉络丛中分离和表征免疫细胞
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Biologia
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JoVE Journal Biologia
Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses
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从微解剖小鼠脉络丛中分离和表征免疫细胞

DOI:
10.3791/63487-v

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09:55 min

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February 03, 2022

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, , , ,
1Brain-Immune Communication Lab,Institut Pasteur, 2Cytometry platform, CB UTechS,Institut Pasteur

Capitoli

  • 00:05Introduction
  • 00:42Dissection of the Choroid Plexus from the Brain
  • 02:47Preparation of Samples for Flow Cytometry Analysis
  • 03:52Choroid Plexus Myeloid Cells Gating
  • 06:46Choroid Plexus T Cells Gating
  • 07:44Results: Myeloid and T Cells Gating Strategy to Identify Choroid Plexus Immune Subsets
  • 09:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

本研究使用流式细胞术和两种不同的门控策略对分离的灌注小鼠脑脉络丛;该协议确定了填充该大脑结构的主要免疫细胞亚群。

Tags

Immune CellsChoroid PlexusesMouse ModelBrain RegulationCell DissectionCell IsolationCell CharacterizationCollagenase IVLateral VentricleThird VentricleFourth VentricleCell DissociationMACS BufferCell CentrifugationCell Washing

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