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用于生物医学研究的3D犬肝和肠类器官培养物的标准化和维护

JoVE Journal
Medicina

This content is Free Access.

JoVE Journal Medicina

Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

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用于生物医学研究的3D犬肝和肠类器官培养物的标准化和维护

DOI:

10.3791/63515-v

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08:39 min

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January 31, 2022

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Vojtech Gabriel, Christopher Zdyrski, Dipak K. Sahoo, Kimberly Dao, Agnes Bourgois-Mochel, Jamie Kopper, Xi-Lei Zeng, Mary K. Estes, Jonathan P. Mochel³, Karin Allenspach³

¹Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, ²Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, ³3D Health Solutions Inc., ⁴Department of Molecular Virology and Microbiology,Baylor College of Medicine

Capitoli

00:04Introduction
00:50Preparation for Isolation
02:03Organoid Isolation
06:15Results: Establishment of 3D Organoid Cultures
08:13Conclusion

Summary

Traduzione automatica

描述了从犬肠和肝脏组织收获成体干细胞以建立3D类器官培养物的实验方法。此外，还讨论了确保持续生长并提供标准操作程序以收获，生物库和恢复犬肠道和肝脏类器官培养的实验室技术。

Tags

Canine Hepatic Organoids Canine Intestinal Organoids Organoid Culture Biomedical Research Standardization Maintenance Isolation Harvesting Biobanking Extracellular Matrix Chelating Solution DMEM Growth Factors

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