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Layer Microdissection of Tricuspid Valve Leaflets for Biaxial Mechanical Characterization and Microstructural Quantification
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JoVE Journal Bioingegneria
Layer Microdissection of Tricuspid Valve Leaflets for Biaxial Mechanical Characterization and Microstructural Quantification

Layer Microdissection of Tricuspid Valve Leaflets for Biaxial Mechanical Characterization and Microstructural Quantification

DOI:
10.3791/63522-v

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07:34 min

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February 10, 2022

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, , ,
1Biomechanics and Biomaterials Design Laboratory (BBDL), School of Aerospace and Mechanical Engineering,The University of Oklahoma, 2Institute for Biomedical Engineering, Science and Technology (IBEST),The University of Oklahoma

Capitoli

  • 00:04Introduction
  • 00:41Microdissection of Tricuspid Valve Leaflet Composite Layers
  • 06:04Results: Histological Assessment and Biaxial Mechanical Testing
  • 07:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes the biaxial mechanical characterization, polarized spatial frequency domain imaging-based collagen quantification, and microdissection of tricuspid valve leaflets. The provided method elucidates how the leaflet layers contribute to the holistic leaflet behaviors.

Tags

MicrodissectionTricuspid Valve LeafletsBiaxial Mechanical CharacterizationMicrostructural QuantificationHeart Valve LeafletsLayer SeparationMechanical TestingMicrostructure Analysis

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