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Assessing Energy Substrate Oxidation In Vitro with 14CO2 Trapping
 

Assessing Energy Substrate Oxidation In Vitro with 14CO2 Trapping

Article DOI: 10.3791/63568-v • 09:20 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1, 1
1Translational Research Program of Pediatric Orthopedics, Department of Surgery, The Children's Hospital of Philadelphia, 2Department of Orthopedic Surgery, Tongji Hospital, Huazhong University of Science and Technology

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This protocol describes an easy-to-use method to examine substrate oxidation by tracking 14CO2 production in vitro.

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Keywords: 14CO2 Trapping Energy Substrate Oxidation Metabolic Flexibility Cell Type-specific Metabolic Disorders Dietary And Pharmacological Interventions Bone Tissue Cell Culture Collagenase Digestion MEM Alpha Medium Trypsin-EDTA Cell Counting Substrate Oxidation Rate
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