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Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

DOI:
10.3791/63623-v

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05:28 min

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May 02, 2022

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, ,
1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University

Capitoli

  • 00:04Introduction
  • 00:28Collection of Microbiota Samples from Mice
  • 01:34DNA Extraction
  • 03:46Sample Preparation for 16S rRNA Gene Sequencing
  • 04:14Results: Comparisons of Gut Microbiota at Genus Level
  • 04:48Conclusion

Summary

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This protocol provides a simple, cost-effective DNA isolation method for the analysis of murine gut microbiota alterations during the development of autoimmune disease.

Tags

Fecal MicrobiotaDNA IsolationCost-effectiveAutoimmune DiseaseMouse ModelGut Microbiota DynamicsLysis BufferPhenol-chloroform ExtractionIsopropanol Precipitation260/280 Ratio

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