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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
 

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Article DOI: 10.3791/63709-v • 09:57 min • July 25th, 2022
July 25th, 2022 •
1, 1, 1, 1, 1, 1, 2, 1,2, 1,2
1Dipartimento di Fisica, Politecnico di Milano, 2Institute for Photonics and Nanotechnologies (IFN-CNR)

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We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.

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Keywords: Stimulated Raman Scattering Microscopy Broadband Vibrational Spectrum Hyperspectral Imaging Label-free Non-invasive PMMA Microbeads Polystyrene Microbeads Dimethyl Sulfoxide Olive Oil Optical Parametric Oscillator Prism Compressor Dispersion Compensation
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