Rivista
/
Bioingegneria
/
Engineering and Characterization of an Optogenetic Model of the Human Neuromuscular Junction
JoVE Journal
Bioingegneria
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Bioingegneria
Engineering and Characterization of an Optogenetic Model of the Human Neuromuscular Junction

Engineering and Characterization of an Optogenetic Model of the Human Neuromuscular Junction

DOI:
10.3791/63759-v

•

11:07 min

•

April 14, 2022

•

, , , ,
1Department of Biomedical Engineering,Columbia University, 2Department of Medicine,Columbia University, 3Gladstone Institutes, 4College of Dental Medicine,Columbia University

Capitoli

  • 00:04Introduction
  • 00:48Fabrication of Bioreactors
  • 02:31Skeletal Muscle Tissue Seeding (Day 3)
  • 03:22Motoneuron Differentiation (Days 0 – 14)
  • 04:01Seeding Motoneuron Aggregates in Bioreactor (Day 14)
  • 05:31Simultaneous Optical Stimulation and Video Recording of Neuromuscular Junction (NMJ) Function (Day 24+)
  • 07:36Batch Processing and Analysis (Day 24+)
  • 08:34Results: Stimulation, Recording, and Analysis of NMJ Function
  • 10:31Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a reproducible, automated, and unbiased imaging system for characterizing neuromuscular junction function using human engineered skeletal muscle tissue and optogenetic motoneurons. This system allows for the functional quantification of neuromuscular connectivity over time and detects diminished neuromuscular function caused by neurotoxins and myasthenia gravis patient serum.

Tags

OptogeneticNeuromuscular JunctionNeuromuscular PathologiesTherapeuticsVideo ProcessingNeurotoxinsMyasthenia GravisPDMSCollagen GelMyoblastsMotoneuron Differentiation

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/63759?article_type=v

Tissue Engineering of the Intestine in a Murine Model
check_url/it/63759?article_type=v

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
check_url/it/63759?article_type=v

Tissue Engineering of a Human 3D in vitro Tumor Test System
check_url/it/63759?article_type=v

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
check_url/it/63759?article_type=v

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
check_url/it/63759?article_type=v

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
check_url/it/63759?article_type=v

Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications
check_url/it/63759?article_type=v

An In Vitro Organ Culture Model of the Murine Intervertebral Disc
check_url/it/63759?article_type=v

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat
check_url/it/63759?article_type=v

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

Read Article