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Bioengineering

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JoVE Journal Bioengineering
Engineering and Characterization of an Optogenetic Model of the Human Neuromuscular Junction
 

Engineering and Characterization of an Optogenetic Model of the Human Neuromuscular Junction

Article DOI: 10.3791/63759-v • 11:07 min • April 14th, 2022
April 14th, 2022 •
1, 1, 1,2, 1,3, 1,2,4
1Department of Biomedical Engineering, Columbia University, 2Department of Medicine, Columbia University, 3Gladstone Institutes, 4College of Dental Medicine, Columbia University

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We describe a reproducible, automated, and unbiased imaging system for characterizing neuromuscular junction function using human engineered skeletal muscle tissue and optogenetic motoneurons. This system allows for the functional quantification of neuromuscular connectivity over time and detects diminished neuromuscular function caused by neurotoxins and myasthenia gravis patient serum.

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Keywords: Optogenetic Neuromuscular Junction Neuromuscular Pathologies Therapeutics Video Processing Neurotoxins Myasthenia Gravis PDMS Collagen Gel Myoblasts Motoneuron Differentiation
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