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Immunology and Infection

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JoVE Journal Immunology and Infection
A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
 

A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19

Article DOI: 10.3791/63787-v • 06:46 min • July 5th, 2022
July 5th, 2022 •
*1, *1,2, 1, 1, 1, 1
1Barbara Davis Center for Diabetes, University of Colorado School of Medicine, 2Department of Endocrinology, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology
* These authors contributed equally

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In line with the urgent need for screening for type 1 diabetes, celiac disease, and coronavirus disease 2019, we developed a high throughput 6-Plex electrochemiluminescence assay to simultaneously detect all four islet autoantibodies, tissue transglutaminase autoantibodies, and antibodies to the receptor binding domain of severe acute respiratory syndrome coronavirus 2.

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Keywords: Multiplex Assay Autoantibody Screening Type 1 Diabetes Celiac Disease COVID-19 GAD65 SARS-CoV-2 RBD IA-2 TTG ZnT8 Proinsulin Biotinylated Antigen Ruthenium-labeled Antigen High-throughput Screening Early Disease Prediction Precise Diagnosis
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