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Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis
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Biologia
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JoVE Journal Biologia
Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis

Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis

DOI:
10.3791/63801-v

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10:46 min

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December 09, 2022

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, , , ,
1Department of Biological Sciences,Louisiana State University, 2Department of Plant Pathology and Crop Physiology,Louisiana State University AgCenter

Capitoli

  • 00:03Introduction
  • 00:53Seed Preparation and Sterilization
  • 01:18Seed Imbibation and Stratification
  • 01:45Seed Sterilization
  • 02:51Seed Plating
  • 03:57Plant Growth and Low CO2 Treatment
  • 04:28Low CO2 Treatment
  • 05:13Confirugre the Fluorescence Imaging System
  • 06:17Measure Quantum Yield of Treated Samples
  • 07:54Opening Data File
  • 08:26Risultati
  • 09:40Conclusions

Summary

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We describe an approach to measure changes in photosynthetic efficiency in plants after treatment with low CO2 using chlorophyll fluorescence.

Tags

Photosynthetic EfficiencyPhotorespiratory MutantsChlorophyll FluorescenceLow CO2 ScreeningSeed PreparationSeed SterilizationPlant GrowthFluorescence ImagingQuantum Yield

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