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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
 

Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

Article DOI: 10.3791/63802-v • 06:13 min • December 9th, 2022
December 9th, 2022 •
*1, 1,2,3, *1
1Department of Bioengineering, Northeastern University, 2Barnett Institute, Northeastern University, 3Department of Biology, Northeastern University
* These authors contributed equally

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In this protocol, we describe how to prepare mammalian cells for single-cell proteomics analysis via mass spectrometry using commercially available reagents and equipment, with options for both manual and automatic pipetting.

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Keywords: Single-cell Proteomics SCoPE2 Mass Spectrometry Freeze-heat Lysis Isobaric Carrier Trypsin Digestion Tandem Mass Tagging LC-MS Analysis
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