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使用SCAD测定可视化疤痕形成 - 一种 非原位 皮肤疤痕测定
JoVE Journal
Biologia
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JoVE Journal Biologia
Visualizing Scar Development Using SCAD Assay – An Ex-situ Skin Scarring Assay
DOI:

07:40 min

April 28, 2022

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Capitoli

  • 00:05Introduction
  • 01:03Scar like Tissue in A Dish (SCAD) Preparation
  • 02:50SCAD: Tissue Culture & Live Imaging of SCADs
  • 04:23Tissue Harvesting and 2D/3D Immunofluorescence Staining
  • 05:07Results: An Ex Situ Skin Scarring Assay to Visualize Scar Development
  • 06:43Conclusion

Summary

Traduzione automatica

该协议描述了称为“培养皿中的SCar样组织”或SCAD的皮肤筋膜外植体的产生。该模型允许在疤痕形成过程中对单个成纤维细胞进行前所未有的可视化。

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