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Capitoli
- 00:04Introduction
- 00:40Loading the Phylogenetic Codon Substitution Frequencies (PhyloCSF) Track Hub to the University of California Santa Cruz (UCSC) Genome Browser
- 01:25Navigating to Genes of Interest Using Gene Identifiers
- 02:20Navigating to Genomic Regions of Interest Using Sequence Information
- 03:10Identifying Conserved Short Open Reading Frames (sORFs) Using PhyloCSF Track Data
- 04:06Viewing Homologous Regions in Other Genomes
- 04:51Generating Multi-Species Sequence Alignments for Microproteins of Interest
- 06:47Results: Identification of Genomic Regions of Interest for Microprotein-Coding Potential
- 08:47Conclusion
Protokollen beskrevet her giver detaljerede instruktioner om, hvordan man analyserer genomiske regioner af interesse for mikroproteinkodningspotentiale ved hjælp af PhyloCSF i den brugervenlige UCSC Genome Browser. Derudover anbefales flere værktøjer og ressourcer til yderligere at undersøge sekvensegenskaber for identificerede mikroproteiner for at få indsigt i deres formodede funktioner.
