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1Putuo People’s Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology,Tongji University, 2National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics,Chinese Academy of Sciences, 3Department of Infectious Diseases, Shanghai Key Laboratory of Infectious Diseases and Biosafety Emergency Response, National Medical Center for Infectious Diseases, Huashan Hospital,Fudan University
Capitoli
- 00:04Introduction
- 00:44Preparation of Myelin Oligodendrocyte Glycoprotein Peptide (MOG35-55) Emulsion and Pertussis Toxin
- 01:55Establishment of Experimental Autoimmune Encephalomyelitis (EAE) Animal Model
- 02:55Open Field Test
- 03:42Analysis of Bone Phenotype
- 04:16Results: Induction of EAE by MOG35-55 Treatment
- 05:49Conclusion
The present protocol describes the induction of experimental autoimmune encephalomyelitis in a mouse model using myelin oligodendrocyte glycoprotein and monitoring the disease process using a clinical scoring system. Experimental autoimmune encephalomyelitis-related symptoms are analyzed using mouse femur micro-computed tomography analysis and open field test to assess the disease process comprehensively.
