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JoVE Journal Bioengineering
Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination
 

Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

Article DOI: 10.3791/63989-v • 09:10 min • June 6th, 2022
June 6th, 2022 •
1,2, 1,2, 1,2, 1,2, 3, 1,2
1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences, Wuyi University

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Cerebral organoids provide unprecedented opportunities for studying organ development and human disease pathology. Although great success has been achieved with cerebral organoid culture systems, there are still operational difficulties in applying this technology. The present protocol describes a cerebral organoid procedure that facilitates medium change and organoid transfer.

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Keywords: Cerebral Organoids Lateral Soft Light Illumination IPSCs EB Formation Anti-adherence Rinsing Solution Rho Kinase Inhibitor Y27632 Centrifugation LED White Lights Low Adhesion Plate Medium Change
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