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Journal / Bioengineering / Handling and Assessment of Human Primary Prostate …
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Bioengineering

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JoVE Journal Bioengineering
Handling and Assessment of Human Primary Prostate Organoid Culture
 

Handling and Assessment of Human Primary Prostate Organoid Culture

Article DOI: 10.3791/59051-v • 08:45 min • January 17th, 2019
January 17th, 2019 •
*1, *1, 1, 1,2,3, 1,3
1Department of Pathology, University of Illinois at Chicago, 2Departments of Urology, Physiology, and Biophysics, University of Illinois at Chicago, 3University of Illinois Cancer Center
* These authors contributed equally

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Here, we present a protocol to guide human primary prostate organoid handling then suggest endpoints to assess phenotype. Seeding, culture maintenance, recovery from matrix gel, morphologic quantification, embedding and sectioning, FFPE sectioning, whole-mount staining, and application of commercial assays are described.

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Keywords: Prostate Organoids In Vitro Culture Primary Cells Matrix Gel Neutral Protease Histology Embedding Precision Medicine Disease Modeling
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