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从不同的脂肪库分离和鉴定血管内皮细胞,用于下游应用
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications
DOI:

09:00 min

June 10, 2022

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Capitoli

  • 00:04Introduction
  • 00:51Tissue Dissection and Cleaning
  • 04:39Digestion of Isolated Arteries for Isolation of Endothelial Cells
  • 06:01Staining of Endothelial Cells Prior to Flow Cytometry
  • 07:06Results: Identification of Endothelial Cells Following Arterial Tissue Digestion
  • 08:15Conclusion

Summary

Traduzione automatica

该协议详细介绍了一种解剖小鼠脂肪库以及分离和消化各自动脉以释放然后鉴定内皮细胞群的方法。用于下游应用的新鲜分离细胞将促进对血管细胞生物学和血管功能障碍机制的理解。

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