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Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes
 

Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes

Article DOI: 10.3791/64169-v • 06:35 min • March 24th, 2023
March 24th, 2023 •
*1, *1,2, 2, 1
1Department of Developmental Biology and Cancer Research, The Institute for Medical Research Israel-Canada, The Hebrew University - Hadassah Medical School, 2Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine
* These authors contributed equally

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Here, we present a protocol to isolate murine intestinal mesenchyme, including telocytes. These can be used for several applications, such as co-culture with mouse or human-derived organoids, to support growth and better reflect the situation in the original tissue.

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Keywords: Murine Intestinal Mesenchyme Telocytes Cell Isolation Tissue Dissociation Intestinal Epithelium Cell-cell Interaction Homeostasis Disease Mesenchymal Signaling Ex Vivo Growth
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