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JoVE Journal Neuroscience
Organotypische retinale Explantatkulturen von Makakenaffen
 
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Organotypische retinale Explantatkulturen von Makakenaffen

Article DOI: 10.3791/64178-v • 10:32 min • August 24th, 2022
August 24th, 2022 •
1, 1, 1, 1, 2, 1
1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University, Yunnan University, 2Institute for Ophthalmic Research, Eberhard-Karls-Universität Tübingen

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Netzhautexplantate von Wildtyp-Makaken wurden in vitro kultiviert. Die Netzhautdegeneration und der cGMP-PKG-Signalweg wurden mit dem PDE6-Inhibitor Zaprinast induziert. Die cGMP-Akkumulation in den Explantaten bei unterschiedlichen Zapinast-Konzentrationen wurde mittels Immunfluoreszenz nachgewiesen.

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Neurowissenschaften Heft 186
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