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JoVE Journal Neuroscience
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
 

Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

Article DOI: 10.3791/64187-v • 11:10 min • July 6th, 2022
July 6th, 2022 •
*1, *2, 3, 1
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine, Jinan University, 2Department of Clinical Pathology, First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine, Florida State University
* These authors contributed equally

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Automatic Translation

Using in ovo electroporation, we devised a method to selectively transfect the auditory inner ear and cochlear nucleus in chicken embryos to achieve a cell-group-specific knockdown of fragile X mental retardation protein during discrete periods of circuit assembly.

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Keywords: In Ovo Electroporation Cell-autonomous Function FMRP Fragile X Syndrome Auditory Circuit Stage-specific Site-specific Direction-specific Fmr1 Knockdown Drug-inducible Vector Embryo Manipulation HH Stage Albumen Removal Windowing Ink Injection
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