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In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window

In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window

DOI:
10.3791/64224-v

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06:45 min

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August 04, 2022

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, , , ,
1Department of Neurophysiology, Faculty of Medicine,University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine,University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine,University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine,The University of Tokyo

Capitoli

  • 00:04Introduction
  • 00:51Cranial Window Construction
  • 03:32Making AAV Film on Plastic Wrap Using Fibroin Solution
  • 04:17Results: Evaluation of Cranial Window Made Using the PVDC Wrap Methods
  • 05:56Conclusion

Summary

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The present protocol describes making a large (6 x 3 mm2) cranial window using food wrap, transparent silicone, and cover glass. This cranial window allows in vivo wide-field and two-photon calcium imaging experiments in the same mouse.

Tags

Cranial WindowWide-field Calcium ImagingTwo-photon Calcium ImagingMouseNeural ActivityGlial ActivityComportamentoMacroscopicMicroscopicPVDC WrapACSFBiological AdhesiveSilicone ElastomerCover GlassFibroinAAV

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