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体内 使用大颅窗的小鼠宽场和双光子钙成像
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window
DOI:

06:45 min

August 04, 2022

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Capitoli

  • 00:04Introduction
  • 00:51Cranial Window Construction
  • 03:32Making AAV Film on Plastic Wrap Using Fibroin Solution
  • 04:17Results: Evaluation of Cranial Window Made Using the PVDC Wrap Methods
  • 05:56Conclusion

Summary

Traduzione automatica

本协议描述了使用食品包装,透明硅胶和盖玻片制作大型(6 x 3 mm2)颅窗。该颅窗允许在同一只小鼠中进行 体内 宽场和双光子钙成像实验。

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