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小鼠胚胎中早期心脏祖细胞的实时成像
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Live Imaging of Early Cardiac Progenitors in the Mouse Embryo
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小鼠胚胎中早期心脏祖细胞的实时成像

DOI:
10.3791/64273-v

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07:02 min

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July 12, 2022

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, , ,
1Cardiovascular Regeneration Program,Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Cardiovascular Development Group, Department of Experimental Biology,University of Jaén, Jaén, 3Fundación Medina, Granada

Capitoli

  • 00:04Introduction
  • 00:41Tools and Holder Customization
  • 02:29Embryo Dissection
  • 03:50Microscope Preparation, Embryo Mounting, and Image Acquisition
  • 05:56Results: 3D Live Imaging of Cardiac Progenitor Cells in the Mouse Embryo
  • 06:35Conclusion

Summary

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我们提出了小鼠胚胎培养和成像的详细方案，可实现心脏祖细胞的3D +时间成像。该视频工具包解决了成功进行实时成像所需的关键技能，否则很难从纯文本出版物中获得。

Tags

Keywords: Live ImagingEarly Cardiac ProgenitorsMouse EmbryoCardiogenesisEmbryo DevelopmentTungsten WireGlass CapillaryPolymethyl MethacrylateStereo MicroscopeUterusDeciduiEctoplacental ConeReichter’s MembraneDissection MediumCulture Medium

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