Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Tyramide Signal Amplification for the…
JoVE Journal
Immunology and Infection

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunology and Infection
Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
 

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Article DOI: 10.3791/64332-v • 09:15 min • October 20th, 2022
October 20th, 2022 •
1,2, 1,2, 1,2, 1,2
1VIB-UGent Center for Inflammation Research, 2Department of Biomedical Molecular Biology, Ghent University

Capitoli

Riepilogo
Automatic Translation

Tyramide signal amplification during immunofluorescent staining enables the sensitive detection of phosphorylated RIPK3 and MLKL during ZBP1-induced necroptosis after HSV-1 infection.

Tags

Tyramide Signal Amplification Immunofluorescent Staining ZBP1-Dependent Phosphorylation RIPK3 MLKL HSV-1 Infection Human Cells Signal Amplification Protocol Necroptosis Induction ZBP1-expressing HT-29 Cells McCoy's 5A Medium Herpes Simplex Virus One Rim Mutant ICP6 Multiplicity Of Infection Necroptosis-inducing Cocktail GSK840 Inhibitor RIPK3 Kinase Activity
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter