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使用成熟的人诱导多能干细胞来源心肌细胞单层进行高通量心脏毒性筛选
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JoVE Journal Bioingegneria
High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers
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使用成熟的人诱导多能干细胞来源心肌细胞单层进行高通量心脏毒性筛选

DOI:
10.3791/64364-v

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14:03 min

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March 24, 2023

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, , , ,
1Frankel Cardiovascular Regeneration Core Laboratory, Cardiovascular Medicine-Internal Medicine,University of Michigan, Ann Arbor, 2CAIRN Research, 3StemBioSys, Inc.

Capitoli

  • 00:04Introduction
  • 00:57Commercially Available Cryopreserved hiPSC‐CMs Plating for Maturation on a Maturation‐Inducing Extracellular Matrix (MECM)
  • 03:02Human‐Induced Pluripotent Stem Cell‐Derived Cardiomyocytes (hiPSC‐CM) Purification
  • 06:01Optical Mapping Using Voltage‐Sensitive Dyes (VSDs) and Calcium‐Sensitive Fluorophores (CSFs)
  • 07:32Optical Mapping Using Genetically Encoded Calcium Indicator (GECI)
  • 08:18Acquisition of Optical Mapping Data and Analysis
  • 10:45Results: Robust and Rapid Maturation of Human Induced Pluripotent Stem Cell‐Derived Cardiomyocytes (hiPSC‐CMs)
  • 13:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

人诱导多能干细胞来源的心肌细胞（hiPSC-CMs）为使用动物进行临床前心脏毒性筛查提供了一种替代方案。hiPSC-CMs在临床前毒性筛查中广泛采用的一个限制是细胞的不成熟，胎儿样表型。这里介绍的是用于hiPSC-CMs稳健和快速成熟的方案。

Tags

Keywords: HiPSC-CMCardiotoxicity ScreeningHigh-throughputMature CardiomyocytesExtracellular MatrixEP AssayMACS

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