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Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
 

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Article DOI: 10.3791/64382-v • 13:11 min • February 10th, 2023
February 10th, 2023 •
1, 1
1Biochemistry & Molecular Biology, University of Texas Medical Branch

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This article provides a straightforward and clear protocol to label Salmonella secreted effectors using genetic code expansion (GCE) site-specifically and image the subcellular localization of secreted proteins in HeLa cells using direct stochastic optical reconstruction microscopy (dSTORM)

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Keywords: Genetic Code Expansion GCE Site-specific Protein Labeling Fluorescent Labeling Zika Virus Super-resolution Imaging Bacterial Secreted Proteins Non-canonical Amino Acids TCO Janelia Fluor 646 Tetrazene BDPFL Tetrazine Confocal Microscopy
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