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一种基于人源酵母模型研究α-突触核蛋白毒性和聚集的方法
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Biologia
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JoVE Journal Biologia
A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
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一种基于人源酵母模型研究α-突触核蛋白毒性和聚集的方法

DOI:
10.3791/64418-v

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08:24 min

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November 25, 2022

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, ,
1Department of Biological Sciences,Ajou University

Capitoli

  • 00:05Introduction
  • 00:48Yeast Transformation and Spotting Assay
  • 02:57Measurement of Yeast Growth Using a Microplate Reader
  • 04:18Fluorescence Microscopy
  • 06:31Results: Cytotoxicity of α-Synuclein on Agar and Liquid Media and Analysis of Yeast Cells by Fluorescence Microscopy
  • 07:28Conclusion

Summary

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需要α-突触核蛋白的 体内 生理模型来研究和了解帕金森病的发病机制。我们描述了一种使用人源化酵母模型监测α-突触核蛋白的细胞毒性和聚集体形成的方法。

Tags

α-synucleinYeast ModelParkinson’s DiseaseProtein AggregationCellular PhenotypesGenetic FactorsGrowth AssayToxicity

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