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Developmental Biology

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JoVE Journal Developmental Biology
Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
 

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Article DOI: 10.3791/64459-v • 10:09 min • September 13th, 2022
September 13th, 2022 •
1,2,3, 1,2, 1,2
1Department of Genetics, Rutgers, The State University of New Jersey, 2Human Genetics Institute of New Jersey, 3Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University

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Error in chromosome segregation is a common feature in oocytes. Therefore, studying the spindle assembly checkpoint gives important clues about the mechanisms needed to produce healthy eggs. The present protocol describes three complementary assays to evaluate spindle assembly checkpoint integrity in mouse oocytes.

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Spindle Assembly Checkpoint SAC Integrity Mouse Oocytes Live Imaging Immunofluorescence Nocodazole Reversine DMSO Oocyte Maturation 96-well Plate Milrinone Culture Media Bright-field Microscope
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