/

Biologia dello sviluppo

/

评估小鼠卵母细胞中纺锤体组件检查点完整性

JoVE Journal
Biologia dello sviluppo

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biologia dello sviluppo

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Please note that all translations are automatically generated. Click here for the English version.

评估小鼠卵母细胞中纺锤体组件检查点完整性

DOI:

10.3791/64459-v

•

10:09 min

•

September 13, 2022

•

Mansour Aboelenain^2,3, Karen Schindler², Cecilia S. Blengini²

¹Department of Genetics,Rutgers, The State University of New Jersey, ²Human Genetics Institute of New Jersey, ³Department of Theriogenology, Faculty of Veterinary Medicine,Mansoura University

Capitoli

00:05Introduction
00:51Nocodazole Treatment and Live Imaging
02:41Monitoring the Pattern of Securin-GFP Degradation During Meiotic Maturation
04:42Recruitment of MAD2 at Kinetochores by Immunofluorescence During Meiotic Maturation
07:26Results: Evaluating Spindle Assembly Checkpoint Integrity in Mouse Oocytes
09:33Conclusion

Summary

Traduzione automatica

染色体分离错误是卵母细胞的常见特征。因此，研究纺锤体组装检查点为生产健康卵子所需的机制提供了重要线索。本协议描述了三种互补测定，以评估小鼠卵母细胞中的纺锤体组装检查点完整性。

Tags

Spindle Assembly Checkpoint Meiosis Oocyte Aneuploidy Live Imaging Immunofluorescence Nocodazole Reversine Securin-GFP Polar Body Extrusion Asymmetrical Cytokinesis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

斑马鱼幼虫骨骼肌诚信与伊文思蓝分析

基于核仁染色质组织分离鼠标窦卵母细胞与表征

马卵母细胞染色体分离，组蛋白乙酰化和主轴形态分析

通过显微注射卵母细胞生成转基因小鼠

小鼠第二腭融合的实时成像

基于神经网络的发育能力或不称职小鼠全生长卵母细胞的识别

有丝分裂过程中染色体分离的活体细胞成像

用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板

小鼠血睾丸屏障完整性的一种活体研究方法

确定小鼠肝脏中的胆汁密度

Read Article