Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

Claudia Manriquez Roman; R. Leo Sakemura; Brooke L. Kimball; Fang Jin; Roman H. Khadka; Mohamad M. Adada; Elizabeth L. Siegler; Aaron J. Johnson; Saad S. Kenderian

doi:10.3791/64535

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Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

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Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

DOI:

10.3791/64535-v

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06:08 min

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February 10, 2023

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Claudia Manriquez Roman^2,3,4,5, R. Leo Sakemura², Brooke L. Kimball², Fang Jin, Roman H. Khadka, Mohamad M. Adada², Elizabeth L. Siegler², Aaron J. Johnson, Saad S. Kenderian^2,6

¹T Cell Engineering Laboratory,Mayo Clinic, Rochester, ²Division of Hematology,Mayo Clinic, Rochester, ³Mayo Clinic Graduate School of Biomedical Sciences,Mayo Clinic, Rochester, ⁴Department of Molecular Medicine,Mayo Clinic, Rochester, ⁵Regenerative Sciences PhD Program,Mayo Clinic, Rochester, ⁶Department of Immunology,Mayo Clinic, Rochester

Capitoli

00:04Introduction
00:49Assessment of CART19 Cell‐Associated Toxicities
01:33MRI Imaging
04:02Results: Assessment of CART Cell‐Associated Toxicities in a PDX Mice Model
05:17Conclusion

Summary

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Here, we describe a protocol in which an acute lymphoblastic leukemia patient-derived xenograft model is used as a strategy to assess and monitor CD19-targeted chimeric antigen receptor T cell-associated toxicities.

Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

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