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部分坐骨神经结扎术:慢性神经性疼痛的小鼠模型,用于研究新疗法的抗伤害感受作用
JoVE Journal
Medicina
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JoVE Journal Medicina
Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies
DOI:

08:16 min

October 06, 2022

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Capitoli

  • 00:05Introduction
  • 00:57First Step: Measure the Baseline of Mechanical Sensitivity Using the Von Frey Test
  • 01:53Second Step: Measure the Baseline of Thermal Sensitivity Using the Hargreaves Test
  • 02:41Measuring the Baseline of Thermal Sensitivity Using the Hot Plate Test
  • 03:47Preoperative Preparation
  • 04:30Surgical Procedure and Postsurgical Behavioral Tests
  • 06:38Results: Dose‐Dependent Reversal of pSNL‐Induced Mechanical Hypersensitivity by Intraperitoneal Injection of Morphine or Ibuprofen
  • 07:36Conclusion

Summary

Traduzione automatica

坐骨神经部分结扎可诱发长期慢性神经性疼痛,其特征是对热和机械刺激的夸张反应。这种神经性疼痛的小鼠模型通常用于研究疼痛管理的创新疗法。本文详细介绍了提高标准化和可重复性的外科手术。

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