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Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
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Biologia
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JoVE Journal Biologia
Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel

Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel

DOI:
10.3791/64563-v

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08:07 min

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December 23, 2022

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, , , , ,
1Research Institute for Health Sciences and Technologies (SABITA),Istanbul Medipol University, 2Cellorama, 3Cancer and Stem Cell Research Center,Maltepe University, 4Department of Histology and Embryology, School of Medicine,Maltepe University, 5Department of Medical Biology, School of Medicine,Maltepe University, 6School of Medicine,Koc University

Capitoli

  • 00:04Introduction
  • 01:14Culturing of the Organoids/Spheroids
  • 02:27Immunofluorescence Labeling of Whole-Mount Organoids/Spheroids in a Hydrogel
  • 04:43Freezing and Thawing of Whole-Mount Organoids/Spheroids in a Hydrogel
  • 05:53Results: Investigating the Whole-Mount Organoids/Spheroids in Hydrogel by Imaging
  • 07:25Conclusion

Summary

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The present study describes methodologies for culturing, freezing, thawing, processing, staining, labeling, and examining entire spheroids and organoids under various microscopes, while they remain intact in a hydrogel within a multipurpose device.

Tags

OrganoidsSpheroidsHydrogel3D CultureFreezingThawingProcessingImagingMicroscopyDisease ModelingBiomarkersHEPG2 CellsImmunofluorescence LabelingFixationWashing

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