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Immunology and Infection
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JoVE Journal Immunology and Infection
Virusvoortplanting en celgebaseerde colorimetrische kwantificering
 
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Virusvoortplanting en celgebaseerde colorimetrische kwantificering

Article DOI: 10.3791/64578-v • 07:26 min • April 7th, 2023
April 7th, 2023 •
1, 1, 2, 1, 1
1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE), Universiti Malaya, 2Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya

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Het huidige protocol beschrijft de verspreiding van het Zika-virus (ZIKV) in niercellen van Vero Afrikaanse groene apen en de kwantificering van ZIKV met behulp van celgebaseerde colorimetrische immunodetectiemethoden in formaten met 24 wells en 96 wells (hoge doorvoer).

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Immunologie en infectie Nummer 194 Zika-virus (ZIKV) Flavivirus Aangeboren hersenafwijkingen Guillain-BarrÃ-syndroom© Virologie Focus Forming Assay (FFA) Viraal Antigeen Peroxidase Immunokleuring Techniek 24-wells formaat 96-wells formaat Foci Size Optimization Vero Cells Incubatie Kleuringsprocessen Celfixatie Permeabilisatie Blokkering Antilichaambinding Peroxidasesubstraat
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