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Generación y obtención de imágenes de organoides epiteliales humanos y de ratón a partir de tejido mamario normal y tumoral sin pasar
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1Department of Internal Medicine, Division of Hematology and Oncology,University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center,University of Texas Southwestern Medical Center, 3Department of Molecular Biology,University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine,University of Texas Southwestern Medical Center, 5Department of Cell Biology,University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine,University of Southern California
Capitoli
- 00:04Introduction
- 00:55Collecting, Digesting Tissue, and Differential Centrifugation
- 04:11Embedding Organoids in BECM and Collagen
- 07:22Results: Immunofluorescent Imaging of Embedded Organoids
- 08:11Conclusion
Este protocolo discute un enfoque para generar organoides epiteliales a partir de tejido mamario primario normal y tumoral a través de centrifugación diferencial. Además, se incluyen instrucciones para el cultivo tridimensional, así como imágenes inmunofluorescentes de organoides incrustados.
