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Generering og billeddannelse af muse- og humane epitelorganoider fra normalt væv og tumorbrystvæv uden passage
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1Department of Internal Medicine, Division of Hematology and Oncology,University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center,University of Texas Southwestern Medical Center, 3Department of Molecular Biology,University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine,University of Texas Southwestern Medical Center, 5Department of Cell Biology,University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine,University of Southern California
Capitoli
- 00:04Introduction
- 00:55Collecting, Digesting Tissue, and Differential Centrifugation
- 04:11Embedding Organoids in BECM and Collagen
- 07:22Results: Immunofluorescent Imaging of Embedded Organoids
- 08:11Conclusion
Denne protokol diskuterer en tilgang til generering af epitelorganoider fra primær normal og tumorbrystvæv gennem differentiel centrifugering. Desuden er instruktioner inkluderet til tredimensionel dyrkning samt immunofluorescerende billeddannelse af indlejrede organoider.
