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小鼠卵巢表观基因组和转录组的细胞特异性配对询问

JoVE Journal
Biologia

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JoVE Journal Biologia

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

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小鼠卵巢表观基因组和转录组的细胞特异性配对询问

DOI:

10.3791/64765-v

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12:25 min

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February 24, 2023

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Sarah R. Ocañas*^1,2,3, José V. V. Isola*^1,3, Tatiana D. Saccon, Kevin D. Pham, Ana J. Chucair-Elliott, Augusto Schneider, Willard M. Freeman³, Michael B. Stout³

¹Aging and Metabolism Research Program,Oklahoma Medical Research Foundation, ²Genes & Human Disease Research Program,Oklahoma Medical Research Foundation, ³Oklahoma City Veterans Affairs Medical Center, ⁴Nutrition College,Federal University of Pelotas

Capitoli

00:04Introduction
01:16Creation of the Nuclei Suspension
04:03Isolation of Nuclei Tagged in Specific Cell Types (INTACT)
06:02Performing Translating Ribosome Affinity Purification (TRAP)
10:02Results: Validation of Cyp17-NuTRAP Mouse Model
11:26Conclusion

Summary

Traduzione automatica

在该协议中，翻译核糖体亲和纯化（TRAP）方法和特定细胞类型（INTACT）方法中标记的细胞核的分离针对使用NuTRAP小鼠模型交叉到Cyp17a1-Cre小鼠系的细胞特异性卵巢转录组和表观基因组的配对询问进行了优化。

Tags

Cell-specific Ovarian Epigenome Ovarian Transcriptome Cell Isolation Cell Type-specific Cre Driver Nuclei Isolation Cell Sorting Paired Analysis Ovarian Aging Female Infertility

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