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Agrobacterium tumefaciens-medieret genetisk transformation af smalbladet plantain
 
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Agrobacterium tumefaciens-medieret genetisk transformation af smalbladet plantain

Article DOI: 10.3791/64777-v • 07:50 min • March 17th, 2023
March 17th, 2023 •
1, 1, 1, 2, 1, 3, 4, 1
1Department of Agronomy, Center for Plant Biology, Purdue University, 2Department of Biology, Purdue University, 3Department of Horticulture, Faculty of Agricultural Sciences, University of the Punjab, 4Department of Botany and Plant Pathology, Center for Plant Biology, Purdue University

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På grund af sin alsidige anvendelse som modelart inden for forskellige fagområder er der behov for et genetisk transformationsværktøjssæt i smalbladet plantain (Plantago lanceolata). Her præsenteres ved hjælp af Agrobacterium tumefaciens-medieret transformation en protokol, der resulterer i stabile transgene linjer med en transformationseffektivitet på 20%.

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Tilbagetrækning nr. 193
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