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マイクロ流体混合で調製したmRNA-脂質ナノ粒子のin vitro および in vivo 効率の試験
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Testing the In Vitro and In Vivo Efficiency of mRNA-Lipid Nanoparticles Formulated by Microfluidic Mixing
DOI:

08:55 min

January 20, 2023

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Capitoli

  • 00:05Introduction
  • 00:50Pre-Formulation Preparation
  • 01:51Preparation of the Lipid and Nucleic Acid Mixes
  • 02:33Microfluidic Formulation of mRNA-LNPs
  • 03:56Post-Formulation Processing and Characterization of the mRNA-LNPs
  • 04:51In Vitro Transfection of HepG2 Cells
  • 05:55In Vivo Evaluation of mRNA-LNPs in Mice Following Tail Vein Injection
  • 07:34Results: Formulation of mRNA-LNPs and the Assessment of In Vitro and In Vivo Performance
  • 08:24Conclusion

Summary

Traduzione automatica

ここでは、ホタルルシフェラーゼをコードするmRNAをカプセル化する脂質ナノ粒子(LNP)を製剤化するためのプロトコルが提示されます。これらのLNPは、in vitro でHepG2細胞で、in vivo でC57BL/6マウスでその効力を試験しました。

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