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Genetics
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Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana
 

Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana

Article DOI: 10.3791/64817-v 11:48 min March 17th, 2023
March 17th, 2023

Capitoli

Riepilogo

A chromatin immunoprecipitation (X-ChIP) assay against the histone mark H3K4me3 for the model organism Exaiptasia diaphana is presented. The specificity and effectiveness of the assay are confirmed by quantitative PCR and next-generation sequencing. This protocol enables the increased investigation of protein-DNA interactions in the sea anemone E. diaphana.

Tags

Keywords: Chromatin Immunoprecipitation ChIP-seq Exaiptasia Diaphana Aiptasia Cnidarian Epigenetics Cross-linking Quenching Lysis Buffer Dounce Tissue Grinder Sonication
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