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体内完整背根神经节中初级感觉神经元网络中神经元集合体的钙成像

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia

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体内完整背根神经节中初级感觉神经元网络中神经元集合体的钙成像

DOI:

10.3791/64826-v

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09:07 min

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February 10, 2023

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John Shannonhouse, Ruben Gomez, Hyeonwi Son, Yan Zhang, Yu Shin Kim²

¹Department of Oral & Maxillofacial Surgery, School of Dentistry,University of Texas Health Science Center at San Antonio, ²Programs in Integrated Biomedical Sciences, Translational Sciences, Biomedical Engineering, Radiological Sciences,University of Texas Health Science Center at San Antonio

Capitoli

00:04Introduction
01:09Surgery and Securing the Animal for Right Side L5 DRG Imaging
03:08DRG Imaging
07:02Results: Imaging Neuronal Population Using Confocal Scanning of Pirt-GCaMP L5 DRG
08:25Conclusion

Summary

Traduzione automatica

该协议描述了背根神经节（DRG）的手术暴露，然后是GCaMP3（基因编码的Ca²⁺ 指示剂;绿色荧光蛋白-钙调蛋白-M13蛋白3）使用Pirt-GCaMP3小鼠对神经元集合进行Ca²⁺ 成像，同时对同侧后爪施加各种刺激。

Tags

Keywords: In Vivo Calcium Imaging Neuronal Ensembles Primary Sensory Neurons Dorsal Root Ganglia Peripheral Allodynia Pain Hypersensitivity Trigeminal Ganglia Geniculate Ganglia Genetically Encoded Sensors Voltage Cell Signaling Molecules Cyclic AMP Surgical Procedure Lumbar Enlargement Transverse Process L5 DRG Isoflurane Anesthesia

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