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Journal / Immunology and Infection / 初代アカゲザルB細胞の遺伝子編集
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Immunology and Infection

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JoVE Journal Immunology and Infection
初代アカゲザルB細胞の遺伝子編集
 
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初代アカゲザルB細胞の遺伝子編集

Article DOI: 10.3791/64858-v • 09:53 min • February 10th, 2023
February 10th, 2023 •
1, 2, 2, 3,5, 1, 1,6, 1, 2, 1,4
1Laboratory of Molecular Immunology, The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Laboratory of Retrovirology, The Rockefeller University, 4Howard Hughes Medical Institute, The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine, King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center, Wistar Institute

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B細胞療法の研究のために、CRISPR/Cas9および組換えアデノ随伴ウイルス血清型6を用いて初代アカゲザルB細胞を培養および遺伝子編集する方法を紹介します。

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今月のJoVE、第192号、B細胞、遺伝子編集、ゲノム工学、CRISPR / Cas9、アデノ随伴ウイルス、アカゲザル、非ヒト霊長類、細胞療法
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