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JoVE Journal Genetics
A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration
 

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

Article DOI: 10.3791/64927-v • 09:24 min • March 10th, 2023
March 10th, 2023 •
1,2, 1, 3, 1,2, 3,4, 1,2
1Department of Medical Genetics, University of Wisconsin-Madison, 2McPherson Eye Research Institute, University of Wisconsin-Madison, 3Department of Ophthalmology, Duke University, 4Department of Cell Biology, Duke University

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Mouse models can be useful tools for investigating the biology of the retinal pigmented epithelium (RPE). It has been established that mice can develop an array of RPE pathologies. Here, we describe a phenotyping protocol to elucidate and quantify RPE pathologies in mice using light, transmission electron, and confocal microscopy.

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Protocol Evaluate Quantify Retinal Pigmented Epithelium Pathologies Mouse Models Age-Related Macular Degeneration RP Pathologies Light Transmission Electron Microscopy Confocal Microscopy Quantitative Assessments Statistical Comparisons Molecular Pathways RPE Health Dissected Mouse Size Practice Gravity Feed Perfusion System Fixative Buffer Syringe Barrel Tubing Line Cardiac Perfusion Euthanized Mouse
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