Rivista
/
Biologia
/
Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
JoVE Journal
Biologia
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

DOI:
10.3791/65106-v

•

09:41 min

•

March 17, 2023

•

,
1Weill Center for Metabolic Health, Department of Medicine,Weill Cornell Medicine

Capitoli

  • 00:04Introduction
  • 01:00Preparation of Buffers and Collection Plates
  • 01:36Preparation of Primary Preadipocyte Samples
  • 02:15Preparation of Ex Vivo Culture Samples
  • 03:44Lipolysis Assay
  • 04:33Free Fatty Acid (FFA) Colorimetric Assay
  • 05:46Glycerol Colorimetric Assay
  • 06:34Calculation of Lipolytic Rate
  • 07:18Results: Basal and Stimulated Lipolytic Rates of In Vitro Differentiated Preadipocytes and Ex Vivo Adipose Tissue
  • 09:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

Triglyceride lipolysis in adipocytes is an important metabolic process resulting in the liberation of free fatty acids and glycerol. Here, we provide a detailed protocol to measure basal and stimulated lipolysis in adipocytes and ex vivo adipose tissue from mice.

Tags

Keywords: LipolysisPreadipocytesMurineEx VivoIn VitroFree Fatty AcidsBSADMEMInsulinDifferentiationDPBSStimulationControlSerial SamplingMeasurementOptimization

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/65106?article_type=v

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
check_url/it/65106?article_type=v

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
check_url/it/65106?article_type=v

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy
check_url/it/65106?article_type=v

Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging
check_url/it/65106?article_type=v

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
check_url/it/65106?article_type=v

Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
check_url/it/65106?article_type=v

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats (Video) | JoVE

check_url/it/65106?article_type=v

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator (Video) | JoVE

check_url/it/65106?article_type=v

Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration (Video) | JoVE

check_url/it/65106?article_type=v

The Significance of Isolation, Culture, and Adipogenic Induction of SVF-Derived Preadipocytes from Mouse Perivascular Adipose Tissue (Video) | JoVE

Read Article